The latest attL fragment was PCR increased from pCMVssattL playing with primers attL(BOP?)Fwd-(KpnI) and you may attL(BOP?)Rev-(NotI)
The latest attL fragment was PCR increased from pCMVssattL playing with primers attL(BOP?)Fwd-(KpnI) and you may attL(BOP?)Rev-(NotI) pTZ-attP4X-UN-EF1?-eGFP try made by subcloning the attP4X succession regarding pattP4X-PGKssPuro once the an enthusiastic EcoRI fragment from the novel EcoRI site upstream of your own individual UTF1 promoter into the pTZ-Us ( dos5) to create pTZ-attP4X-Un. The new EF1?-eGFP succession is actually PCR-increased...